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human embryonic kidney 293 cell line hek 293t  (ATCC)
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ATCC human embryonic kidney 293 cell line hek 293t
Human Embryonic Kidney 293 Cell Line Hek 293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic kidney 293 cell line hek 293t - by Bioz Stars, 2026-02
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human embryonic kidney 293 t cell line  (ATCC)
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ATCC human embryonic kidney 293 t cell line
Human Embryonic Kidney 293 T Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic kidney 293 t cell line/product/ATCC
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human embryonic kidney 293 t cell line - by Bioz Stars, 2026-02
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293 t human embryonic kidney cell line  (ATCC)
99
ATCC 293 t human embryonic kidney cell line
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
293 T Human Embryonic Kidney Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/293 t human embryonic kidney cell line/product/ATCC
Average 99 stars, based on 1 article reviews
293 t human embryonic kidney cell line - by Bioz Stars, 2026-02
99/100 stars
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human embryonic kidney cell line 293  (ATCC)
99
ATCC human embryonic kidney cell line 293
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Human Embryonic Kidney Cell Line 293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic kidney cell line 293/product/ATCC
Average 99 stars, based on 1 article reviews
human embryonic kidney cell line 293 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human embryonic kidney epithelial cell line hek 293  (ATCC)
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ATCC human embryonic kidney epithelial cell line hek 293
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Human Embryonic Kidney Epithelial Cell Line Hek 293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic kidney epithelial cell line hek 293/product/ATCC
Average 99 stars, based on 1 article reviews
human embryonic kidney epithelial cell line hek 293 - by Bioz Stars, 2026-02
99/100 stars
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human embryonic kidney cell lines hek293  (ATCC)
99
ATCC human embryonic kidney cell lines hek293
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Human Embryonic Kidney Cell Lines Hek293, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic kidney cell lines hek293/product/ATCC
Average 99 stars, based on 1 article reviews
human embryonic kidney cell lines hek293 - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
human embryonic kidney 293 hek293 cell lines  (ATCC)
99
ATCC human embryonic kidney 293 hek293 cell lines
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Human Embryonic Kidney 293 Hek293 Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic kidney 293 hek293 cell lines/product/ATCC
Average 99 stars, based on 1 article reviews
human embryonic kidney 293 hek293 cell lines - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
ap 1 luciferase reporter human embryonic kidney 293 recombinant cell line  (BPS Bioscience)
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BPS Bioscience ap 1 luciferase reporter human embryonic kidney 293 recombinant cell line
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Ap 1 Luciferase Reporter Human Embryonic Kidney 293 Recombinant Cell Line, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ap 1 luciferase reporter human embryonic kidney 293 recombinant cell line/product/BPS Bioscience
Average 93 stars, based on 1 article reviews
ap 1 luciferase reporter human embryonic kidney 293 recombinant cell line - by Bioz Stars, 2026-02
93/100 stars
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human embryonic kidney epithelial cell line  (ATCC)
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ATCC human embryonic kidney epithelial cell line
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Human Embryonic Kidney Epithelial Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human embryonic kidney epithelial cell line/product/ATCC
Average 99 stars, based on 1 article reviews
human embryonic kidney epithelial cell line - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
cell lines human embryonic kidney cell line hek293t cells  (ATCC)
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ATCC cell lines human embryonic kidney cell line hek293t cells
Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of <t>293</t> <t>T</t> cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group
Cell Lines Human Embryonic Kidney Cell Line Hek293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cell lines human embryonic kidney cell line hek293t cells/product/ATCC
Average 99 stars, based on 1 article reviews
cell lines human embryonic kidney cell line hek293t cells - by Bioz Stars, 2026-02
99/100 stars
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Image Search Results


Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of 293 T cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group

Journal: Journal of Nanobiotechnology

Article Title: RVG-targeted extracellular vesicles loaded with echinatin attenuate dopaminergic neurodegeneration via the IGF-2/PI3K/Akt pathway in Parkinson’s disease mice

doi: 10.1186/s12951-025-03997-5

Figure Lengend Snippet: Preparation and characteristics of RVG-EVs@Echi. ( a ) Schematic illustration of the preparation process of RVG-EVs@Echi. ( b ) Representative fluorescence microscopy images of 293 T cells transduced with RVG lentivirus showing the infection efficiency. Scale bar: 200 nm. ( c ) Representative TEM image (inset) and size distribution of RVG-EVs@Echi. Scale bar: 100 nm. ( d ) Size distribution of free EVs and RVG-EVs. ( e ) Representative confocal images and ( f ) statistical analysis results showing the targeted delivery of RVG-EVs to neurons. sEVs are labeled with DiI (red), and neurons are labeled with alpha-tubulin (green). Nuclei are labeled with DAPI (blue). Scale bars, 50 μm for the original images and 10 μm for the magnified images. ( g ) Echi loading efficiency after room temperature incubation or electroporation. ( h ) Zeta potential of RVG-EVs and RVG-EVs@Echi, as measured by DLS. ( i ) Size distribution of RVG-EVs@Echi in PBS or in 20% FBS over time. ( j ) Free-EVs@Echi and RVG-EVs@Echi release curves of Echi over time. ( k ) Echi leakage rate of RVG-EVs@Echi over time. ( l ) Representative flow cytometry histograms and ( m ) quantitative time-course analysis of uptake (mean fluorescence intensity, MFI) by flow cytometry in MN9D cells incubated with DiI-labeled Free EVs or RVG-EVs for 3, 6, or 12 h. Data are shown as mean ± SEM. ** p < 0.01 vs. the Free EVs group

Article Snippet: The 293 T human embryonic kidney cell line (acquired from ATCC) was cultured in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS; Invitrogen).

Techniques: Fluorescence, Microscopy, Transduction, Infection, Labeling, Incubation, Electroporation, Zeta Potential Analyzer, Flow Cytometry